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Histochemistry and Fluorescent MicroscopyMicroglia were identified using lectin histochemistry. For immunohistochemistry and fluorescent microscopy, brain sections were blocked, incubated in primary antibodies overnight at 4 followed by a biotinylated anti rat IgG (1:2,000; Vector Laboratories) or Cy3 conjugated goat anti rabbit IgG(H (1:200, 56,399; Jackson Immuno Research, West Grove, PA, USA) at room temperature for 1 respectively (Han et al, 2003). For immunohistochemistry, proteins were detected using the Elite Vectastain ABC kit (Vector Laboratories) and diaminobenzidine, then counterstained with hematoxylin and eosin.

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That’s a tough one. Thanks very much. We’re joined but our security analyst, Matthew Olson.

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2016 Stone island small logo patch t shirt in white