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Of all the surviving texts mentioning cosmetics (all written by men) Ovid is alone in his approval of their use. The consensus was that women who used cosmetics in excess were immoral and deceptive and were practicing a form of witchcraft. Juvenal wrote that “a woman buys scents and lotions with adultery in mind” and mocked the need for cosmetics, believing that they were ineffective.
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Histochemistry and Fluorescent MicroscopyMicroglia were identified using lectin histochemistry. For immunohistochemistry and fluorescent microscopy, brain sections were blocked, incubated in primary antibodies overnight at 4 followed by a biotinylated anti rat IgG (1:2,000; Vector Laboratories) or Cy3 conjugated goat anti rabbit IgG(H (1:200, 56,399; Jackson Immuno Research, West Grove, PA, USA) at room temperature for 1 respectively (Han et al, 2003). For immunohistochemistry, proteins were detected using the Elite Vectastain ABC kit (Vector Laboratories) and diaminobenzidine, then counterstained with hematoxylin and eosin.
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That’s a tough one. Thanks very much. We’re joined but our security analyst, Matthew Olson.
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