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Output from CD HIT was converted to mothur format (Schloss et al., 2009) with Perl, and community similarity trees and Venn diagrams were constructed in mothur. Sequences were aligned against a template alignment from the Silva rRNA database project for phylogenetic analysis (Pruesse et al., 2007) using the mothur alignment package. Trees were built with maximum likelihood and neighbour joining algorithms in ARB (Ludwing et al., 2004) using a 75 homology filter.
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